|
Media
à
----------
Org.
ß
|
Trans-
port
Media.
|
Nutrient
Agar
(Ordinary
Media)
(NA)
|
Blood
Agar
(BA) /
Chocholate
Agar (CA)
|
Mac-
Conkey
Medium
(Lactose-ferm’n)
|
Selective /
Enriched /
Enrichment
Media.
|
Remarks
|
|
Staph.
Aureus/Pyogenes
& Staph
Albus / Epidemidis
|
|
Golden Yellow
Colonies
|
b-Hemolysis
(Complete)
(BA)
|
Pink Colonies
(+ve)
|
--Black colonies on K-Tellurite media.
--Ludlam’s medium( salt
milk agar)
|
|
|
Strepto.
Pyogenes
(Group A)
|
|
|
a
/
b Hemolysis
( BA )
a-hemolysis
strepto.viridans
|
|
|
Dick test
for Scarlet-fever
|
|
Strepto.pneumoniae
|
|
|
a-Hemolysis
Greenish discolouration around
colonies.
|
|
|
Carrom Coin app.
-Quellung
reaction.-capsule
|
|
N.meningitidis
Meningococci
|
|
|
BA / CA used
Translucent
Convex colonies
|
|
|
|
|
N.gonorrhoea
Gonococci
|
Stuart’s
Transport media
|
|
BA / CA used
Translucent
Round colonies
|
|
Thayer-Martin
Media ( with
Vancomycin, Nystatin, Colistin )
|
5-10%CO2
( Candle jar used )
|
|
Cornyb.
diptheria
|
|
|
BA
|
|
--Loeffler’s Serum slope
--K-Tellurite Blood agar
(0.04%)
( Macleod & Hoyle’s
media)
|
Albert’s Stain for Metachromatic granules.
Shick-test( diphteriasusceptibility )
Elek’s gel ppt. Test & Guinea pig inoculation—for
Toxigenicity / virulence.
|
|
Mycobacteria
TB.
|
|
|
|
|
--Lowenstein Jensen(L.J.)
solid media.
--Dubos Liquid media
--Loeffler’s serum slope
--Petroff’s medium
--Dorset’s Egg
medium
|
-Auramine & Rhodamine staining for Fluorescent
microscopy.
-NAP test +ve, Niacin
test & Neutral Red test +ve
|
|
Mycobacteria
Leprae.
|
|
|
|
|
--Foot Pad of Mice ( 20oC)
--Nine Branded Armadillo.
--Human Foetal spinal Ganglion
Cells
|
Do not grow in cell
free culture
|
|
Spirochaetes.
|
|
|
|
|
-No cultivation
--Only Dark Ground Microscopy
of Smears .
|
Fontanas Method
(smears)
Levaditi
Staining
(tissue section)
Nichol’s Strain ( Rabbit Testes)
|
|
Chlamydia Trachomatis.
|
|
|
|
|
-Do not grow in cell-free media.
--obligate intracellular org.
|
-Inclusion bodies
in Giemsa stain smears
--ELISA.
|
|
Salmonella Typhi
|
|
NA.
|
|
Colourless
Colonies.
|
-Selenite F &
- Tetrathionate broth.
-Deoxycholate citrate agar
-Wilson&Blair
medium(black colonies-s.typhi&s.paratyphi B.)
--Blood culture-Ist.wk.+ve—TSB-
Trypsicase soy broth
-Bile broth
Castaneda’s double medium.
|
Widal testà
(+ve at end of 1st
wk.)
‘O’titre
> 1: 100
& ‘H’titer
> 1:200
is significant.
NOTE:-
BASU
1 2 3 4 wks.
B-blood
Culture
A-Antibody / WIDAL estm’n
S-stool
Culture
U-urine culture
|
|
Vibrio
Cholerae
|
1) VR-
venkatraam-ramakrishnan medium
2) Clary Blair medium
|
NA
|
BA-Partial
Hemolysis
( greening)
( eltor)
|
Colourless
colonies
|
1) Alkaline peptone water-surface pellicle
2) BSA-alkaline Bile Salt
Agar,ph8.0)
3) TCBS medium-
Thiosulphate,citrate,bromthymaol
bule & sucrose,
4) Monsur’s GTTA medium.
|
-String test
-Hanging drop prep.-to see motility.
-strains-
Ogawa
Inaba
Hikojima
-Cholera red reaction-
nitroso-indole forma’n
|
|
Shigella
|
|
|
|
Colourless colonies
Except—Sh.sonnei( late lactose ferm’n)
|
-Deoxycholate citrate agar
|
|
|
E-coli
|
|
|
|
Pink
colonies
|
|
|
|
Klebsella
|
|
|
|
Pink
colonies
|
|
|
|
Proteus
|
|
NA
( Swarming Growth)
|
|
Colourless colonies
|
Tetrathionate Broth
|
Swarming inhibited by 6% Agar & Sodium Azide.
--PPA test +ve
(deamination test)
|
|
Pseudomonas
|
|
NA
( Greenish colonies—Pyocanin)
|
|
Colourless
|
|
Pigment produced are—Pyocyanin, Fluroscin, Pyoverdin, Pyomelanin.
|
|
Clostridium welchii
|
Stuart’s transport medium
& Thioglycollate
broth.
|
|
|
|
Robertson’s cooked meat medium
( this medium turns PINK)
|
-Subterminal spores
-Lecithinase effect—Nagler
reaction.
|
|
Cl.Tetani
|
Stuart’s transport medium
& Thioglycollate
broth.
|
|
|
|
Robertson’s cooked meat medium
( this medium turns BLACK)
|
-Central spores
Drum stick app.
|
|
Citrobcater,
Enterobacter,
Serratia
|
|
|
|
Pink colonies
|
|
|
|
|
|
|
|
|
|
|