O

R

G

A

N    

I

S

M

C

O

A

G

U

L

ASE

P

H

OS

P

H

AT

AS

E

F

E

R

ME

N

TA

‘N

C

A

TA

L

AS

E

O

X

I

D

A

S

E

Solubility

In 10%Bile

Bacitracin/Optocin

Sensitivity

Nitrate

Reduc’n

test

I

N

D

O

L

E

formation

UREASE

test

Methyl Red

Test

MR

Voges-Proskauer tset

VP

Citrate

Test

PPA

test

Staph.

Aureus

/Pyogenes

+ve

+ve

Mannitol ( Acid only)

+ve

Staph

Albus / Epidemidis

-ve

-ve

+ve

Staph.

saprophyticus

-ve

+ve

Strepto.

Pyogenes

(Group A)

-ve

-v

e

Not soluble

Bacitracin

Sensitive

Strepto.

pneumoniae

Inulin( Acid only)

-ve

-v

e

Soluble in 10%Bile

Optocin

Sensitive

N.meningitidis

Meningococci         

Glucose& Maltose( Acid only)

+ve

+ve

N.gonorrhoea

Gonococci

Glucose(Acid only )

+ve

+ve

Cornyb.

diptheria

Glucose,Maltose,Galactose( Acid only )

Mycobacteria

TB.

NRT+ve

NAP+ve

Niacin +ve

Neutral Red test +ve

Mycobacteria

Leprae

Spirochaetes.

Chlamydia Trachomatis.

Salmonella Typhi

Glucose&Mannitol ( Acid & Gas-H2S)TSI-test+ve

+ve

Vibrio

Cholerae

Glucose,Mannitol,Maltose&Sucrose

+ve

+ve

+ve

NitrosoIndole formed =

+ve

Cholera Red

Reaction

-ve

-ve

VP

test,Hemolysis,Chick RBCs Aggluttination

+ve

in

Eltor Vibrios

Shigella

Mannitol Ferm’n Except-Sh.Dysenteriae & Lactose is fermented only by Sh.Sonnei.

+ve

-ve

+ve

-ve

E-coli

Glucose ( with Gas)

-v

e

+ve

-ve

+ve

Klebsella

?+ve

+ve

+ve

Proteus

+ve(imp)

+ve

Deamination Reaction

Phenylalanineàphenylpyruvic acid

Pseudomonas

+ve

+ve

+ve

Clostridium welchii

Glucose,Lactose,Maltose,Sucrose(acid & gas-H2S)TSI-test+ve

Litmustest+ve(Stomy ferm’n)

+ve

-ve

+ve

-ve

Cl.Tetani

-ve

+ve

-ve

-ve

Citrobcater,

Enterobacter,

Serratia

?+ve

?+ve

+ve

+ve